Tuesday, April 7, 2015

Study group discussion: Fluoride bulb and glycolysis

Best agent to be added into blood sample that has to be transported for glucose estimation?

Fluoride!

It prevents glycolysis.

How does it prevents glycolysis?

Inhibits the enzyme enolase

Yeah, correct!

Why do we use fluoride bulb for glucose estimation?
Fluoride inhibits glycolysis. So the glucose in RBCs don't get metabolized and you won't get a false lower value of glucose (:

This is the note I took on fluoride! Apparently when coupled with Mg it ihhibits enolase.. So it stops glycolysis.

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