Tuesday, April 20, 2021

Venepuncture (Blood collection)

 Venepuncture (Blood collection)
Cotton, ethanol, Syringe (size according to the amount of blood to be collected), needle (blue 23G or purple 24G), Vacutainers 

• Take verbal consent of patient. 
• Follow universal precautions. 
• Ask patient if blood had been collected recently. If yes, try to avoid that site. 

Contraindications of venepuncture from a particular site:
1) IV fluids being given to the patient at the same time 
2) local signs of infection 
3) Haematoma 
4) fistula (created for dialysis)
- Paralyzed is avoided 

• Hand of the patient should be supported. 
• Apply tourniquet (generally a glove is used to tie around the arm instead) and ask the patient to open and close the fist a few times (for congestion of vein). 
• Do not tap the veins. 
• Palpate for vein (For direction and course of vein).
 • Clean the area with cotton and alcohol in a circular motion from in-to-out without re-cleaning the area once it has been cleaned. 
• Do not re-palpate.

• Stretch the skin with non-dominant hand and pierce the skin with the needle (with bevelled end facing up) at an angle of 20 degree. 
• Aspirate the blood into the syringe.  
• Before removing the needle, untie the tourniquet (it helps to reduce the blood loss after retracting the needle)

• Remove the site. 
• Recap needle while putting pressure with the help of cotton swab on it by placing the cap on a surface and then insert needle attached to syringe inside the cap (Recapping is generally not advisable but commonly practised). 
• After capping it properly, remove the needle along with its cap. 
• Then push the blood from the syringe into prelabelled vacutainers by removing their covers. 
• Thank the patient for cooperation.

To collect venous blood for venous blood gas analysis, when heparin isn't available, do the following:
1) Collect blood in green vacutainer
2)Attach a new needle to the syringe
3)Aspirate blood from the vacutainer

FOR BLOOD CULTURE: 8-10ml blood is drawn into the syringe through a sterile needle and then pushed out into a blood  culture bottle containing the transport medium. 
The specimen is refrigerated immediately (although immediate transport of specimen to laboratory is preferred.) 

Written by our guest author - Abhishek Ganacharya
Illustrations by Anveshi Nayan



  1. Thanks you soo much for such great explanations ��������

  2. Are you sure you must avoid blood collection from a previous venepucture site? Infact the previous phlebotomy marks will serve as a guide for subsequent pricks and avoid multiple veins being fired up


This is express yourself space. Where you type create something beautiful! <3
Wondering what do I write? Well...
Tell us something you know better. You are a brilliant mind. Yes, you are! ^__^
Ask about something you don't understand @_@?
Compliment... Say something nice! =D
Be a good critic and correct us if something went wrong :|
Go ahead. Comment all you like here! (:

PS: We have moderated comments to reduce spam. ALL comments that are not spam will be published on the website.