Venepuncture (Blood collection)
REQUIREMENTS:
Cotton, ethanol, Syringe (size according to the amount of blood to be collected), needle (blue 23G or purple 24G), Vacutainers
PROCEDURE:
• Take verbal consent of patient.
• Follow universal precautions.
• Ask patient if blood had been collected recently. If yes, try to avoid that site.
Contraindications of venepuncture from a particular site:
1) IV fluids being given to the patient at the same time
2) local signs of infection
3) Haematoma
4) fistula (created for dialysis)
- Paralyzed is avoided
• Hand of the patient should be supported.
• Apply tourniquet (generally a glove is used to tie around the arm instead) and ask the patient to open and close the fist a few times (for congestion of vein).
• Do not tap the veins.
• Palpate for vein (For direction and course of vein).
• Clean the area with cotton and alcohol in a circular motion from in-to-out without re-cleaning the area once it has been cleaned.
• Do not re-palpate.
• Stretch the skin with non-dominant hand and pierce the skin with the needle (with bevelled end facing up) at an angle of 20 degree.
• Aspirate the blood into the syringe.
• Before removing the needle, untie the tourniquet (it helps to reduce the blood loss after retracting the needle)
• Remove the site.
• Recap needle while putting pressure with the help of cotton swab on it by placing the cap on a surface and then insert needle attached to syringe inside the cap (Recapping is generally not advisable but commonly practised).
• After capping it properly, remove the needle along with its cap.
• Then push the blood from the syringe into prelabelled vacutainers by removing their covers.
• Thank the patient for cooperation.
To collect venous blood for venous blood gas analysis, when heparin isn't available, do the following:
1) Collect blood in green vacutainer
1) Collect blood in green vacutainer
2)Attach a new needle to the syringe
3)Aspirate blood from the vacutainer
FOR BLOOD CULTURE: 8-10ml blood is drawn into the syringe through a sterile needle and then pushed out into a blood culture bottle containing the transport medium.
The specimen is refrigerated immediately (although immediate transport of specimen to laboratory is preferred.)
VIDEO LINK:
Written by our guest author - Abhishek Ganacharya
Illustrations by Anveshi Nayan
#Ae(ONE)INTERN
Thanks you soo much for such great explanations ��������
ReplyDeleteAre you sure you must avoid blood collection from a previous venepucture site? Infact the previous phlebotomy marks will serve as a guide for subsequent pricks and avoid multiple veins being fired up
ReplyDelete